SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). #N207) LAL Reagent Water (Lonza cat. Awareness Technology Inc. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. 2. FlexStation® 3 Multi-Mode Microplate Reader. MOLECULAR DEVICE M5. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. 2 Chemical Reagents 1. 340 nm / 80 nm. SpectraMAX 340 plate reader (Molecular Devices). 4; 100 m M NaCl, 0. 305 reference level saturation. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. plates can be used in the SPECTRAmax PLUS 384. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. Our absorbance plate readers feature our PathCheck Sensor technology and. 04. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. or microplate reader (e. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Acceptor: 665nm. Other technologies such as imaging. Quick View. Heme was preincubated for 5 min with A when added before the metals. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Hydrolysis of compounds 9-15 was monitoredAbsorption was measured at 405 nm against 490 nm using a microplate reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Molecular Devices SpectraMAX 340 Microplate Reader. 5 - 2. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. ). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 6) and 0. 00 USD. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Immunofluorescence and Aggregation Analysis. Also for: Spectramax 190, Spectramax 340pc384, Versamax. Percent Transmission Absorbance Competitor 0. Example: [Drawer]. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Molecular Devices Corporation 1311. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. 1% PEG 8000 at 37°C. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. 340 nm / 70 nm. 5 stars - 1602 reviewsThis assay typically develops in 1. Intra- and inter-assay coefficients of variation were lower than 8. SpectraMax Multi-Mode Microplate Readers. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 0, MSD) or Spectramax 340 (Molecular Devices). All data were normalized to experimental day 0. Microplate Readers. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. 20. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. SpectraMax 384 Plus. SoftMax Pro. plates can be used in the SPECTRAmax PLUS 384. . Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. Data System. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. In this study we used tetanus toxoid (TT) as a well-defined antigen to. 30. 0 to 4. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. The VersaMax and SpectraMax 190 read 96-well microplates. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). The sensitivities of the hormones are Prolactin: 0. Acceptor: 665/10 nm. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. Microplate Spectrophotometer Operator’s Manual. Each experiment was performed in duplicate. Read one sample or up to 384. 313 reference gain check fail. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). 2. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. chen@case. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). SpectraMAX 340. Statistical analysis. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). Molecular Devices SPECTRAmax. The light features a slim profile to prevent. Manager: Yinghua Chen, PhD Contact Email: yinghua. Not. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The contents of the. 7. Italic and boldface type are used for emphasis. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. VersaMax reads 96-well and strip-well plates. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. 0. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. SpectraMax ® Plus 384 optics. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Microplate Reader Shelf. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Stat Fax 4700 Microstrip Reader. mums. 3 0. control or celatrol-treated cells. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. Details. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 4. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. 1. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Water – distilled water or equivalent. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). 6. If depot service is the best option for you we will assist you with logistics to help you keep. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). Dissolve 13. Statistical analysis. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). SoftMax Pro. The VersaMax and SpectraMax 190 read 96-well microplates. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Amino Acid Analysis. Unit powers. used. using SpectraMax i3 (Molecular devices, San Jose, USA). 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. , Sunnyvale, CA, USA) at a wavelength of 450 nm. Donor: 616/10 nm. reader (Molecular Devi ces). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Briefly, cells were seeded into a. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Samples were analyzed in triplicates. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. 6 (h) x 22. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Male 8 pin mini Din to Female 9D. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Details. Microplate Readers. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. chen@case. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. 0% and ± 0. To measure the cell biomass (g/L),. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Page: 1/6. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). , Sunnyvale, CA) at 490 nm. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Description 1. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. 1 mL of culture media with 10% FBS. Cell permeabilization. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. Amino Acid Analysis. Includes: Computer Loaded with Softmax Pro 5. ). Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 3. Number of flashes. 0 3. 313 reference gain check fail. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. Analysis of ALA by Ehrlich’s Reagent Assay. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 00. SpectraMAX 340. In vivo anti-malarial assay in mice. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. HTRF-compatible SpectraMax Multi-Mode Readers. Data were expressed as the mean LPS-treated cells (100%) vs. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). 314 low lamp level warning. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. g. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. Service Contracts, Preventative Maintenance and Performance. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. Molecular Devices Spectramax 340PC instructions. Briefly, cells were sequentially treated with terminal deoxynucleotidyl. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Assay setup for a 384-well low-volume plate. ATI/240, 250. A 0. control. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. These isotypes cause antigen cross-linking by multimeric binding and self. Microplate Spectrophotometers. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. MCE has not independently confirmed the accuracy of these methods. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. One plate carrier adapter is provided with the instrument. Product Keyword. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 2 software was used to measure and calculate of the bioactivity activity studies. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Cell proliferation was measured using the MTT method. G. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. The initial rate of hydrolysis was determined using the early linear region of. Rate of fibrinolysis. 5. Near bottom. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. 5 mL of 1 M acetate buffer (pH 4. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Details. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. Microplate Readers. SpectraMax QuickDrop Micro-Volume Spectrophotometer. ), which was proportional to the number of damaged cells. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Please see photos. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Products : SpectraMax Plus 384 reader,. (7. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. edu Contact Phone: 216-368-8962. Plates were washed with 1× KP solution and blocked with. (1993). The reaction was stopped with 1 M hydrochloric acid after 15 min. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. SpectraMax Multi-Mode Microplate Readers. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. Wavelength Selection. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. reader (SpectraMax 340; Molecular devices, LLc). The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. ScanLater Western Blot Detection Cartridge. Samples containing 25 μg of protein were separated on 12. , Sunnyvale, CA, USA) at 595 nm. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. , Sunnyvale, Calif. chen@case. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. Or scan up to 384 wells at any time to determine the best assay conditions. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. Subsequent replacements require only the lamp (N-34-LB) be replaced. 316 grating motor driver faulty. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Calculating % of total mediator release in RBL assay. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. MCE has not independently confirmed the accuracy of these methods. Supplier: Molecular Devices. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Six wells were assayed for each set of conditions and stadard deviations. SpectraMax Multi-Mode Microplate Readers. Isolation of RNA and RT-PCR. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Vessel Sprout Formation Assay. Binding affinity for denosumab. Bertin Precellys Evolution Touch Homogenizer. SPECTRAMAX 340. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). One plate carrier adapter is provided with the instrument. PerkinElmer. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. The. 5. 4) was followed according to Vanacker et al. 06 ng/mL; FSH: 0. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. Integration delay. Immunofluorescence and Aggregation Analysis. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. TDP-43 stress granular cells were treated with 70% ice-cold. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. The reaction mixture was incubated for 5 min. Each SpectraMax® 340 PC 384 Microplate Reader has an. Call 1-800-745-2710. DNA extraction from blood meal ELISA homogenate.